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KMID : 1101320120440030112
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Korean Journal of Clinical Laboratory Science
2012 Volume.44 No. 3 p.112 ~ p.117
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Development of an Escherichia coli Biofilm Model on Transwell
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Kim Bok-Yung
Thyiam General
Kang Ji-Eun
Lee Seung-Hwan
Park Sang-Hee
Kim Jung-Sun
Abraham Marion
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Abstract
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Escherichia coli biofilm, reported to be produced in the human intestine causing a significant health risk, was successfully grown on transwell?. This biofilm layer was identified by crystal violet staining and prepared for the in vitro E. coli biofilm system which can be used to screen for inhibitors. The biofilm formation did not show a change in transepithelial electrical resistance values. Furthermore, rhodamine 123 staining showed that the dye did not pass through the membrane once biofilm was formed.
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KEYWORD
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Escherichia coli, biofilm, rhodamine 123, crystal violet, transepithelial electrical resistance
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